Antioxidant and Antibacterial Activity of Acetone Extract of Javanese Pepper Fruit (Piper retrofractum Vahl.)

Iqbal Musthapa, Gun Gun Gumilar

Sari


Javanese Pepper (Piper retrofractum Vahl.) has been known as economically valuable herb and has been traditionally used as folk medicine (jamu) against various kinds of health disorders, such as bronchitis, fever, tuberculosis, diarhea, as mouthwash, and aphrodisiac. Until now, there is no report about antioxidant and antibacterial activity of acetone extract of Javanese pepper fruit originated from West Java. Therefore, this study analyzes antioxidant and antibacterial activities of acetone extract of Javanese pepper fruit originated from West Java. Antioxidant activity determined by DPPH (1,1-diphenyl-2-picrilhydrazil) radical scavenging method, while antibacterial activity determined by agar diffusion method with Eschericia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans as test bacterials. Acetone extract of Javanese pepper fruit is proven to possess antioxidant activity with IC50 value of 825 ppm. Meanwhile, antibacterial activity determination of acetone extract of Javanese pepper fruit shows inhibition zone on Staphylococcus aureus, but no inhibition found for another tested bacterials.

Kata Kunci


Javanese Pepper; Antioxidant; Antibacterial

Teks Lengkap:

PDF (English)

Referensi


F. Rahmadani, “Uji Aktivitas Antibakteri dari Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea coromandelica) Terhadap Bakteri Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Pseudomonas aeruginosa,” Skripsi, Universitas Islam Negeri Syarif Hidayatullah, Jakarta, 2015.

Siswanto, “Saintifikasi Jamu Sebagai Upaya Terobosan Untuk Mendapatkan Bukti Ilmiah Tentang Manfaat dan Keamanan Jamu”, Buletin Penelitian Sistem Kesehatan, vol. 15, no. 2, pp. 203-211, 2012.

T.K. Lim, Edible Medicinal and Non-Medicinal Plants, Volume 4, Dordrecht: Springer, 2012. [Buku Elektronik] Tersedia: Springer.

B.T.T. Luyen, B.H. Tai, N.P. Thao, S.Y. Yang, N.M. Cuong, Y.I. Kwon, H.D. Jang dan Y.H. Kim, “A new phenylpropanoid and an alkylglycoside from Piper retrofractum leaves with their antioxidant and a-glucosidase inhibitory activity,” Bioorganic & Medicinal Chemistry Letters, vol. 24, no. 17, pp. 4120-4124, 2014.

Y. Jamal, P. Irawati, A. Fathoni and A. Agusta, “Chemical Constituents and Antibacterial Effect of Essential Oil of Javanese Pepper Leaves (Piper retrofractum Vahl.),” Media Litbangkes, vol. 23, no. 2, pp. 65-72, 2013.

K. Heyne, Tumbuhan Berguna Indonesia Jilid III. Jakarta: Badan Litbang Kehutanan, 1987.

H.S. Bodiwala, G. Singh, R. Singh, C.S. Dey, S.S. Sharma, K.K. Bhutani dan I.P. Singh, “Antileishmanial amides and lignans from Piper cubeba and Piper retrofractum”, Journal Natural Medicine, vol. 61, no. 4, 418-421, 2007.

K.J. Kim, M.S. Lee, K. Jo dan J.K. Hwang, “Piperidine alkaloids from Piper retrofractum Vahl. Protect against high-fat diet-induced obesity by regulating lipid metabolism and activating AMP-activated protein kinase,” Biochemical and Biophysical Research Communications, vol. 411, no. 1, pp. 219-225, 2011.

U. Chansang, N.S. Zahiri, J. Bansiddhi, T. Boonruad, P. Thongsrirak, J. Mingmuang, N. Benjapong, M.S. Mulla, “Mosquito larvicidal activity of aqueous extracts of long pepper (Piper retrofractum Vahl.) from Thailand.” Journal of Vector Ecology, vol. 30, no. 2, pp. 195-200, 2006.

M. Kubo, R. Ishii, Y. Ishino, K. Harada, N. Matsui, M. Akagi, E. Kato, S. Hosoda dan Y. Fukuyama, “Evaluation of Constituents of Piper retrofractum Fruits on Neurotrophic Activity,” Journal of Natural Products, vol. 76, no. 4, pp. 769-773, 2012.

R. Aspan, Sherley, R. Napitupulu, L.S. Wisaksono, Efizal, L. Mooduto, T. Herawaty, A. Novianti, S. Wahyu dan Tumino, Taksonomi Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. Jakarta: BPOM Republik Indonesia, 2008.

R. Muharini, Z. Liu, W. Lin dan P. Proksch, “New amides from the fruits of Piper retrofractum”, Tetrahedron Letters, vol. 56, no. 19, pp. 2521-2525, 2015.

A.E.Z. Hasan, M.K. Suryani, A. Setiyono dan J.J. Silip, “Antiproliferation activities of Indonesian java chili, Piper retrofractum Vahl., against breast cancer cells (MCF-7)”, Der Pharmacia Lettre, vol. 8, no. 18, 141-147, 2016.

M.P. Prasad, S. Shekhar dan B. Amit, “Phytochemical Analysis and Antioxidant potential of Piper species and its Molecular Characterization by RAPD Markers”, International Journal of Fundamental & Applied Sciences, vol. 1, no. 1, pp. 7-10, 2012.

E. Conde, A. Moure, H. Domínguez dan J.C. Parajó, “Extraction of natural antioxidants from plant foods” dalam Separation, Extraction and Concentration Processes in the Food, Beverage and Nutraceutical Industries, S.S.H. Rizvi, Eds. Woodhead Publishing Series in Food Science, Technology and Nutrition, 2013. [Buku Elektronik] Tersedia: Elsevier.

K. Das, R.K.S. Tiwari dan D.K. Shrivastava, “Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends”, Journal of Medicinal Plants Research, vol. 4, no. 2, pp. 104-111, 2010.

TT. Cushnie, B. Cushnie dan A.J. Lamb, “Alkaloids: An overview of their antibacterial, antibiotic enhancing and antivirulence activities”, International Journal of Antimicrobial Agents, vol. 44, no. 5, pp. 377-386, 2014.

M. Arabski, A. Węgierek-Ciuk, A., G. Czerwonka, A. Lankoff dan W. Kaca, “Effects of Saponins against Clinical E. coli Strains and Eukaryotic Cell Line”, Journal of Biomedicine and Biotechnology, vol. 2012, 1-6, 2012.

A.K. Jaiswal, N. Abu‐Ghannam dan S. Gupta, “A comparative study on the polyphenolic content, antibacterial activity and antioxidant capacity of different solvent extracts of Brassica oleracia vegetables,” International Journal of Food Science & Technology, vol. 47, no. 2, pp. 223-231, 2012.

[21] Nouri, L., Nafchi, A.M. and Karim, A.A., “Phytochemical, antioxidant, antibacterial, and α-amylase inhibitory properties of different extracts from betel leaves”, Industrial Crops and Products, vol. 62, 47-52, 2014.

B.S. Furniss, A.J. Hannaford, P.W.G. Smith, A.R. Tatchell, Vogel's Textbook of Practical Organic Chemistry, 5th ed., London: Longman Scientific & Technical, 1989.

M. Alfarabi, M. Bintang dan M. Safithri, “The Comparative Ability of Antioxidant Activity of Piper crocatum in Inhibiting Fatty Acid Oxidation and Free Radical Scavenging”, HAYATI Journal of Biosciences, vol. 17, no. 4, pp. 201-204, 2010.

E.L.D. Kamto, D.S.B. Ngono, J.N. Mbing, A. de Théodore Atchadé, D.E. Pegnyemb dan J.H. van der Westhuizen, “An Aromatic Amide C-glycoside and A Cyclitol Derivative from Stem Barks of Piper guineense Schum and Thonn (Piperaceae)”, Phytochemistry Letters, vol. 10, pp. lxxvi-lxxxi, 2014.

Putri, “Profil Fisikokimia Buah Cabe Jawa (Piper retrofractum V.)”, Skripsi, Universitas Pendidikan Indonesia, Bandung, 2016.

P. Mandal, S.S. Babu dan N.C. Mandal, “Antimicrobial activity of saponins from Acacia auriculiformis”, Fitoterapia, vol. 76, no. 5, pp. 462-465, 2005.

A. Marifah, “Efek Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Pertumbuhan Bakteri Staphylococcus aureus,” Skripsi, Universitas Islam Negeri Syarif Hidayatullah, Jakarta, 2012

U. Taukoorah, N. Lall dan F. Mahomoodally, “Piper betle L. (betel quid) shows bacteriostatic, additive, and synergistic antimicrobial action when combined with conventional antibiotics”, South African Journal of Botany, vol. 105, pp. 133-140, 2016.

G.M. Costa, E.H. Endo, D.A.G. Cortez, T.U. Nakamura, C.V. Nakamura dan B.P. Dias Filho, “Antimicrobial effects of Piper hispidum extract, fractions and chalcones against Candida albicans and Staphylococcus aureus”, Journal de Mycologie Medicale, vol. 26, no. 3, pp. 217-226, 2016.

M.T. Murtiwi, “Aktivitas Antibakteri Ekstrak Etanol Daun Macaranga tanarius (L.) Mull. Arg. Terhadap Streptococcus pyogenes ATCC 19615,” Skripsi, Universitas Sanata Dharma, 2014.




DOI: https://doi.org/10.17509/ci.v1i1.33183

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##