DIVERSITY OF ENDOFIT BACTERIA Vetiveria zizanioides (WILD TYPE) AND ITS POTENTIAL AS AN ANTIBACTERIAL

Abstract

Research has been carried out on molecular characterization and identification leaf endophytic bacteria Vetiveria zizanioides (wild type). The aim of this research was to characterize and identify the morphology of leaf endophytic bacteria V.zizanioides (wild type). Observation of the characteristics of the endophytic bacteria V.zizanioides (wild type) carried out in terms of morphology, cell shape and Gram type, antibacterial activity test, and biochemical activity tests which include starch hydrolysis, lipid hydrolysis, hydrolysis tests casein, gelatin hydrolysis, carbohydrate fermentation, catalase test, H2S production test, test motility, and the IMViC test (indole test, methyl red test, Voges-Proskauer test, and citrate test). After molecular identification, isolates ability to inhibit the growth of Escherichia coli and Pseudomonas aeruginosa and Staphylococcus aureus was tested. Results suggested that VD3, VD4, and VD8 have the ability to inhibit growth of E. coli and P. aeruginosa and isolates VD13 and VD14 inhibited S. aureus growth.