Qualitative Analysis of the Effect of Cattle Housing Conditions on the Protein Content of Raw Milk Using the Biuret Method

Abizar Al Akhdan, Bunga Fatima Ramadhani, Muhammad Ihsan Maulana, Raya Amalia Nugroho, Sabrina Anindita Febrianti, Didik Prinyandoko, Diah Kusumawati, Nurfitri Yulianty, Restu Utari Dewina

Abstract


Whole cow’s milk is a source of animal protein whose content can be influenced by environmental factors, such as temperature, altitude, feed type, and barn sanitation. This study aims to compare the protein content of raw cow’s milk from the Lembang and Pangalengan regions in West Java using the Biuret method for qualitative analysis. Samples were collected from six locations: Maribaya, Parongpong, Cikole, Margamekar, Margamukti, and Jalan Raya Pangalengan. Test results showed that all samples tested positive for protein, indicated by the formation of a purple color. Samples from Lembang produced a darker purple color with an absorbance of approximately 8,000 ppm, while samples from Pangalengan showed a lighter purple color with an absorbance of 1,000–4,000 ppm. These results indicate that differences in the cows’ living conditions can affect milk protein content. The Biuret method proved effective for detecting protein in raw cow’s milk.


Keywords


Bandung; Biuret Test; Cattle Living Conditions; Protein Content; Whole Cow’s Milk

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References


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DOI: https://doi.org/10.17509/jobs.v3i1.102775

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