Influences of Size and Amount of Colloidal Template and Droplet Diameter on the Formation of Porous-structured Hyaluronic Acid Particles

Asep Bayu Dani Nandiyanto, Kikuo Okuyama


Combination of spray drying and templating method has been reported as one of the best method to produce porous pharmaceutical particles. However, current development reports have shown successful formation of porous particles with improper information on what mechanism occurs during the synthesis process and what process parameters involve the creation of particles with controllable pore structures and morphologies. In this report, influences of spray-drying parameters (i.e., size and amount of colloidal template, and droplet diameter) on porous particle fabrication were investigated. Hyaluronic acid (as a model of pharmaceutical drug host component) and polystyrene spheres (as a model of template) were used. The experimental results showed that the spray-drying parameters strongly influenced the external morphology (i.e., spherical and doughnut-shaped), particle outer diameter (from 300 to 2000 nm), and porous configuration (i.e., number of hole cavities and pore sizes (from 100 to 300 nm)) of particles. To qualitatively explain our synthesis strategies to get porous structure, the proposal of the particle formation mechanism was added.


Hyaluronic acid; Polymer template; Spray method; Porous; Doughnut

Full Text:



Faure, A., York, P., & Rowe, R. C. (2001). Process control and scale-up of pharmaceutical wet granulation processes: a review. European Journal of Pharmaceutics and Biopharmaceutics, 52(3), 269-277.

He, M., Zhao, Z., Yin, L., Tang, C., & Yin, C. (2009). Hyaluronic acid coated poly (butyl cyanoacrylate) nanoparticles as anticancer drug carriers. International Journal of Pharmaceutics, 373(1), 165-173.

Iskandar, F., Nandiyanto, A. B. D., Widiyastuti, W., Young, L. S., Okuyama, K., & Gradon, L. (2009). Production of morphology-controllable porous hyaluronic acid particles using a spray-drying method. Acta Biomaterialia, 5(4), 1027-1034.

Maa, Y. F., Nguyen, P. A., Andya, J. D., Dasovich, N., Sweeney, T. D., Shire, S. J., & Hsu, C. C. (1998). Effect of spray drying and subsequent processing conditions on residual moisture content and physical/biochemical stability of protein inhalation powders. Pharmaceutical research, 15(5), 768-775.

Mumenthaler, M., Hsu, C. C., & Pearlman, R. (1994). Feasibility study on spray-drying protein pharmaceuticals: Recombinant human growth hormone and tissue-type plasminogen activator. Pharmaceutical Research, 11(1), 12-20.

Nandiyanto, A. B. D., Kim, S. G., Iskandar, F., & Okuyama, K. (2009). Synthesis of spherical mesoporous silica nanoparticles with nanometer-size controllable pores and outer diameters. Microporous and Mesoporous Materials, 120(3), 447-453.

Nandiyanto, A. B. D., Hagura, N., Iskandar, F., & Okuyama, K. (2010). Design of a highly ordered and uniform porous structure with multisized pores in film and particle forms using a template-driven self-assembly technique. Acta Materialia, 58(1), 282-289.

Nandiyanto, A. B. D., & Okuyama, K. (2011). Progress in developing spray-drying methods for the production of controlled morphology particles: From the nanometer to submicrometer size ranges. Advanced Powder Technology, 22(1), 1-19.

Nandiyanto, A. B. D., Suhendi, A., Arutanti, O., Ogi, T., & Okuyama, K. (2013). Influences of surface charge, size, and concentration of colloidal nanoparticles on fabrication of self-organized porous silica in film and particle forms. Langmuir, 29(21), 6262-6270.

Nandiyanto, A. B. D., Suhendi, A., Ogi, T., Umemoto, R., & Okuyama, K. (2014). Size-and charge-controllable polystyrene spheres for templates in the preparation of porous silica particles with tunable internal hole configurations. Chemical Engineering Journal, 256, 421-430.

Nandiyanto, A. B. D., Sofiani, D., Permatasari, N., Sucahya, T. N., Wiryani, A. S., Purnamasari, A., Rusli, A., & Prima, E. C. (2016). Photodecomposition profile of organic material during the partial solar eclipse of 9 march 2016 and its correlation with organic material concentration and photocatalyst amount. Indonesian Journal of Science and Technology, 1(2), 132-155.

Ogi, T., Nandiyanto, A. B. D., & Okuyama, K. (2014). Nanostructuring strategies in functional fine-particle synthesis towards resource and energy saving applications. Advanced Powder Technology, 25(1), 3-17.

Pavanetto, F., Conti, B., Genta, I., & Giunchedi, P. (1992). Solvent evaporation, solvent extraction and spray drying for polylactide microsphere preparation. International Journal of Pharmaceutics, 84(2), 151-159.

Vanbever, R., Mintzes, J. D., Wang, J., Nice, J., Chen, D., Batycky, R., & Edwards, D. A. (1999). Formulation and physical characterization of large porous particles for inhalation. Pharmaceutical Research, 16(11), 1735-1742.




  • There are currently no refbacks.

Copyright (c) 2017 Asep Bayu Dani Nandiyanto, Kikuo Okuyama

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Lisensi Creative Commons

Indonesian Journal of Science and Technology is published by UPI Press. This work is licensed under a Creative Commons Attribution-Sharealike 4.0.

StatCounter - Free Web Tracker and Counter View My Stats