Betel Leaf Extract’s Effect (Piper Batle Linn) on The Healing Process of Incision Wounds in Rats (Rattus Norvegicus)

Idola Perdana Sulistyoning Suharto, Eva Dwi Ramayanti, Endang Mei Yunalia, Novia Ulfa

Abstract


ABSTRACT.

Introduction: An incision wound is a wound that is intentionally made by a clean-cut using a sharp object. Betel leaf is a natural ingredient that helps the wound healing process because it has active ingredients such as saponins, tannins, flavonoids, essential oils, and alkaloids. Objectives: The study aimed to determine the effect of betel leaf extract on the healing process of incision wounds in white rats. Methods: This study uses a true experimental research design conducted on white rats. Samples were taken by random sampling and divided into four groups, namely control group (KK) (n = 6), treatment group with a dose of 10% (P1) (n = 6), treatment group with a dose of 15% (P2) (n = 6) and treatment group with a dose of 20% (P3) ( n = 6). Results: The mean of wound’s length in KK=0.78 cm, P1=0.67 cm, P2=0.57 cm, and P3=0.15. Based on the Kruskal Wallis test, p value=0.000(α<0.05), there is a significant difference in the wound healing process between the control and treatment groups. Discussion: Betel leaf contains active substances, namely flavonoids, alkaloids, saponins, tannins, and essential oils. The active substance causes the wound’s edges to close faster, so the wound heals faster.

ABSTRAK

Pendahuluan : Luka sayatan adalah luka yang sengaja dibuat dengan sayatan bersih menggunakan benda tajam. Daun sirih merupakan bahan alami yang membantu proses penyembuhan luka, karena memiliki bahan aktif seperti saponin, tanin, flavonoid, minyak atsiri dan alkaloid. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun sirih terhadap proses penyembuhan luka sayatan pada tikus putih. Metode : Penelitian ini menggunakan rancangan penelitian true eksperimental yang dilakukan pada tikus putih. Sampel diambil secara random sampling dan dibagi menjadi empat kelompok yaitu kelompok kontrol (KK) (n = 6), kelompok perlakuan dengan dosis 10% (P1) (n = 6), kelompok perlakuan dengan dosis 15% ( P2) (n = 6) dan kelompok perlakuan dengan dosis 20% (P3) ( n = 6). Hasil : Hasil penelitian menunjukkan bahwa rerata panjang luka pada KK=0,78 cm, P1=0,67 cm, P2=0,57 cm, dan P3=0,15. Berdasarkan uji Kruskal Wallis didapatkan nilai p=0,000(α<0,05), yang berarti terdapat perbedaan yang signifikan dalam proses penyembuhan luka antara kelompok kontrol dan perlakuan. Pembahasan : Daun sirih mengandung zat aktif yaitu flavonoid, alkaloid, saponin, tanin, dan minyak atsiri. Zat aktif tersebut menyebabkan tepi luka lebih cepat menutup, sehingga luka lebih cepat sembuh.


Keywords


Betel leaf extract; wound healing; incision

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References


Agrawal, A. (2018) ‘Unified classification of open fractures: Based on Gustilo and OTA classification schemes’, injury, 49(8), pp. 1526–1531. doi: 10.1016/j.injury.2018.06.007.

Alamgir, A. N. M. (2017) Herbal drugs: Their collection, preservation, and preparation; evaluation, quality control, and standardization of herbal drugs, Progress in Drug Research. doi: 10.1007/978-3-319-63862-1_10.

Anheyer, D. et al. (2018) ‘Herbal Medicine in Children With Respiratory Tract Infection: Systematic Review and Meta-Analysis’, Academic Pediatrics, 18(1), pp. 8–19. doi: 10.1016/j.acap.2017.06.006.

Bauer, S. (2013) ‘Flexible electronics: Sophisticated skin’, Nature Materials, 12(10), pp. 871–872. doi: 10.1038/nmat3759.

Berardesca, E., Farage, M. and Maibach, H. (2013) ‘Sensitive skin: An overview’, International Journal of Cosmetic Science, 35(1), pp. 2–8. doi: 10.1111/j.1468-2494.2012.00754.x.

Darmawan, A. et al. (2021) ‘Betel Leaf Extract Efficacy on Wound Healing : A Systematic review’, STRADA Jurnal Ilmiah Kesehatan, 10(1), pp. 526–536. doi: 10.30994/sjik.v10i1.666.

Etika, A. N., Nurrahayu, K. I. and Suharto, I. P. S. (2017) ‘Effect of Ginger Extract (Zingiber Officinale Roscoe) on the Number of Fibroblast Cells in Rats (Rattus Norvegicus)’, Journal of Nursing Care & Biomolecular, 2(1), pp. 10–14.

Feng, C. J. et al. (2019) ‘Adipose-derived stem cells-induced burn wound healing and regeneration of skin appendages in a novel skin island rat model’, Journal of the Chinese Medical Association, 82(8), pp. 635–642. doi: 10.1097/JCMA.0000000000000134.

Gilaberte, Y. et al. (2016) Anatomy and Function of the Skin, Nanoscience in Dermatology. Elsevier Inc. doi: 10.1016/B978-0-12-802926-8.00001-X.

Górniak, I., Bartoszewski, R. and Króliczewski, J. (2019) Comprehensive review of antimicrobial activities of plant flavonoids, Phytochemistry Reviews. doi: 10.1007/s11101-018-9591-z.

Herryawan, H. and Sabirin, I. P. R. (2018) ‘The effectiveness of red betel leaf (Piper crocatum) extract against periodontal pathogens’, Bali Medical Journal, 7(3), pp. 732–735. doi: 10.15562/bmj.v7i3.1173.

Jenks, P. J. et al. (2014) ‘Clinical and economic burden of surgical site infection (SSI) and predicted financial consequences of elimination of SSI from an English hospital’, Journal of Hospital Infection, 86(1), pp. 24–33. doi: 10.1016/j.jhin.2013.09.012.

Liu, K. et al. (2020) ‘Short-Term Effect of Different Taping Methods on Local Skin Temperature in Healthy Adults’, Frontiers in Physiology, 11(May). doi: 10.3389/fphys.2020.00488.

Liu, Y. et al. (2020) ‘Natural indole-containing alkaloids and their antibacterial activities’, Archiv der Pharmazie, 353(10), pp. 1–10. doi: 10.1002/ardp.202000120.

Madhumita, M., Guha, P. and Nag, A. (2020) ‘Bio-actives of betel leaf (Piper betle L.): A comprehensive review on extraction, isolation, characterization, and biological activity’, Phytotherapy Research, 34(10), pp. 2609–2627. doi:10.1002/ptr.6715.

Mazumder, S., Roychowdhury, A. and Banerjee, S. (2016) ‘An overview of Betel leaf (Piper betle L.): A review’, Annals: Food Science and Technology, 17(2), pp. 367–376.

Misiakos, E. P. et al. (2014) ‘Current Concepts in the Management of Necrotizing Fasciitis’, Frontiers in Surgery, 1(September), pp. 1–10. doi: 10.3389/fsurg.2014.00036.

Mohanty, C. and Sahoo, S. K. (2017) ‘Curcumin and its topical formulations for wound healing applications’, Drug Discovery Today, 22(10), pp. 1582–1592. doi: 10.1016/j.drudis.2017.07.001.

Payne-James, J. (2015) Injury, Fatal and Nonfatal: Sharp and Cutting-Edge Wounds, Encyclopedia of Forensic and Legal Medicine: Second Edition. Elsevier Ltd. doi: 10.1016/B978-0-12-800034-2.00223-8.

Peddapalli, H. et al. (2020) ‘Therapeutic potential of Piper betle : An amazing nature’s medicinal reservoir’, Chemistry Research Journal, 5(3), pp. 62–75.

Prescott, S. L. et al. (2017) ‘The skin microbiome: Impact of modern environments on skin ecology, barrier integrity, and systemic immune programming’, World Allergy Organization Journal, 10(1), pp. 1–16. doi: 10.1186/s40413-017-0160-5.

Roy, A. and Guha, P. (2018) ‘Formulation and characterization of betel leaf (Piper betle L.) essential oil based nanoemulsion and its in vitro antibacterial efficacy against selected food pathogens’, Journal of Food Processing and Preservation, 42(6), pp. 1–7. doi: 10.1111/jfpp.13617.

Shedoeva, A. et al. (2019) ‘Wound healing and the use of medicinal plants’, Evidence-based Complementary and Alternative Medicine, 2019(Figure 1). doi: 10.1155/2019/2684108.

Suharto, I. P. . and Etika, A. . (2019) ‘Ginger Extract (Zingiber Officinale Roscoe) Affects Incision Wound Collagen Fiber Density’, Care, 7(1), pp. 27–36. doi: 10.1088/0256-307X/36/3/030302.

Suharto, I., Rahayu, K. and Etika, A. (2017) ‘The Effect of Ginger (Zingiber Officinale Roscoe) Extract to the Number of Neutrophil Cell in Incision Wound of White Rats (Rattus Norvegicus)’, Advances in Health Sciences Research, 2, pp. 381–385. doi: 10.2991/hsic-17.2017.59.

Sulistyoning Suharto, I. P. et al. (2021) ‘The Effect of the Mahkota Dewa (Phaleria macrocarpa) on the Density of Collagen Incision Wounds’, Journal of Physics: Conference Series, 1899(1). doi: 10.1088/1742-6596/1899/1/012071.

Tirayo, A. J., Munir, M. A. and Hutasoit, G. A. (2016) ‘The Comparison Of Inhibitory Effect Between Antiseptic Soap With Betel Leaf Extract ( Piper Betle Linn ) On The Growth Of Escherichia Coli’, Jurnal Ilmiah Kedokteran, 3(3), pp. 31–39. Available at: http://jurnal.untad.ac.id/jurnal/index.php/MedikaTadulako/article/view/9273.

Yang, C., Wu, H. and Wang, J. (2019) ‘Formulation and evaluation of controlled-release of steroidal saponins-loaded collagen microspheres’, Materials Technology, 34(9), pp. 534–539. doi: 10.1080/10667857.2019.1591727.

Zhao, Y. et al. (2020) ‘Antibacterial activity of tea saponin from Camellia oleifera shell by novel extraction method’, Industrial Crops and Products, 153(February), p. 112604. doi: 10.1016/j.indcrop.2020.112604.




DOI: https://doi.org/10.17509/jpki.v7i2.40359

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