How to Read and Interpret FTIR Spectroscope of Organic Material

Asep Bayu Dani Nandiyanto, Rosi Oktiani, Risti Ragadhita


Fourier Transform Infrared (FTIR) has been developed as a tool for the simultaneous and quantitative determination of organic components, including chemical bond, as well as organic content (e.g. protein, carbohydrate and lipid). However, until now, there is no further information for the detailed information in the FTIR peaks. The objective of this study was to demonstrate how to read and assess chemical bond and structure in the organic material. The analysis was then compared with the literatures. The step-by-step method on how to read the FTIR data was presented, including reviewing simple to the complex organic materials. This study is potential to be used as a standard information on how to read FTIR peaks in the biochemical and organic materials.


FTIR; infrared spectrum; organic material; chemical bond; organic structure

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Agarwal, R., Tandon, P., and Gupta, V. D. (2006). Phonon dispersion in poly (dimethylsilane). Journal of Organometallic Chemistry, 691(13), 2902-2908.

Andrus, P. G., and Strickland, R. D. (1998). Cancer grading by Fourier transform infrared spectroscopy. Biospectroscopy, 4(1), 37-46.

Andrus, P. G. (2006). Cancer monitoring by FTIR spectroscopy. Technology in cancer research and treatment, 5(2), 157-167.

Argov, S., Sahu, R. K., Bernshtain, E., Salman, A., Shohat, G., Zelig, U., and Mordechai, S. (2004). Inflamatory bowel diseases as an intermediate stage between normal and cancer: A FTIR-microspectroscopy approach. Biopolymers: Original Research on Biomolecules, 75(5), 384-392.

Barry, B. W., Edwards, H. G. M., and Williams, A. C. (1992). Fourier transform Raman and infrared vibrational study of human skin: assignment of spectral bands. Journal of Raman spectroscopy, 23(11), 641-645.

Chiang, H. P., Song, R., Mou, B., Li, K., Chiang, P., Wang, D., Tse, W., and Ho, L. (1999). Fourier transform Raman spectroscopy of carcinogenic polycyclic aromatic hydrocarbons in biological systems: Binding to heme proteins. Journal of Raman spectroscopy, 30(7), 551-555.

Chiriboga, L., Xie, P., Yee, H., Vigorita, V., Zarou, D., Zakim, D., and Diem, M. (1998). Infrared spectroscopy of human tissue. I. Differentiation and maturation of epithelial cells in the human cervix. Biospectroscopy, 4(1), 47-53.

Coates, J. (2000). Interpretation of infrared spectra, a practical approach. Encyclopedia of analytical chemistry, 12, 10815-10837.

Desi, I. R. (2016). Isolasi dan Karakterisasi Senyawa Alkaloid dari Cacing Tanah (Lumbricus Rubellus Hoffmeister) (Doctoral dissertation, Fakultas MIPA (UNISBA)).

Dovbeshko, G., Chegel, V., Gridina, N. Y., Repnytska, O., Shirshov, Y., Tryndiak, V., Todor, I., and Solyanik, G. (2002). Surface enhanced IR absorption of nucleic acids from tumor cells: FTIR reflectance study. Biopolymers: Original Research on Biomolecules, 67(6), 470-486.

Dovbeshko, G. I., Gridina, N. Y., Kruglova, E. B., and Pashchuk, O. P. (2000). FTIR spectroscopy studies of nucleic acid damage. Talanta, 53(1), 233-246.

Eckel, R., Huo, H., Guan, H. W., Hu, X., Che, X., and Huang, W. D. (2001). Characteristic infrared spectroscopic patterns in the protein bands of human breast cancer tissue. Vibrational Spectroscopy, 27(2), 165-173.

Fabian, H., Jackson, M., Murphy, L., Watson, P. H., Fichtner, I., and Mantsch, H. H. (1995). A comparative infrared spectroscopic study of human breast tumors and breast tumor cell xenografts. Biospectroscopy, 1(1), 37-45.

Fan, M., Dai, D., and Huang, B. (2012). Fourier transform infrared spectroscopy for natural fibres. In Fourier transform-materials analysis: InTech.

Fujioka, N., Morimoto, Y., Arai, T., and Kikuchi, M. (2004). Discrimination between normal and malignant human gastric tissues by Fourier transform infrared spectroscopy. Cancer Detection and Prevention, 28(1), 32-36.

Fukuyama, Y., Yoshida, S., Yanagisawa, S., and Shimizu, M. (1999). A study on the differences between oral squamous cell carcinomas and normal oral mucosas measured by Fourier transform infrared spectroscopy. Biospectroscopy, 5(2), 117-126.

Fung, M. F. K., Senterman, M. K., Mikhael, N. Z., Lacelle, S., and Wong, P. T. (1996). Pressure-tuning fourier transform infrared spectroscopic study of carcinogenesis in human endometrium. Biospectroscopy, 2(3), 155-165.

Huleihel, M., Salman, A., Erukhimovitch, V., Ramesh, J., Hammody, Z., and Mordechai, S. (2002). Novel spectral method for the study of viral carcinogenesis in vitro. Journal of biochemical and biophysical methods, 50, 111-121.

Istiqomah, L., Sofyan, A., Damayanti, E., and Julendra, H. (2009). Amino acid 7. profile of earthworm and earthworm meal (Lumbricus rubellus) for animal feedstuff. Journal of the Indonesian Tropical Animal Agriculture, 34(4), 253-257.

Moore S, Spackman D. H, and Stein W. H. (1958). Chromatography of amino 8. acids on sulfonated polystyrene resins. An improved system. Anal Chem, 30(7), 1185-1190.

Jaggi, N., and Vij, D. (2006). Fourier transform infrared spectroscopy. In Handbook of Applied Solid State Spectroscopy. Boston: Springer, 411-450.

Kirk, R. E., and Othmer, D. F. (1953). Encyclopedia of Chemical Technology Vol. 2. The Interscience Encyclopedia, Inc; New York.

Lucassen, G. W., Van Veen, G. N., and Jansen, J. A. (1998). Band analysis of hydrated human skin stratum corneum attenuated total reflectance Fourier transform infrared spectra in vivo. Journal of biomedical optics, 3(3), 267-281.

Mordechai, S., Mordehai, J., Ramesh, J., Levi, C., Huleihal, M., Erukhimovitch, V., Moser, A., and Kapelushnik, J. (2001). Application of FTIR microspectroscopy for the follow-up of childhood leukemia chemotherapy. Subsurface and Surface Sensing Technologies and Applications, 3, 243-251.

Mordechai, S., Sahu, R. K., Hammody, Z., Mark, S., Kantarovich, K., Guterman, H., Podshyvalov, J., Goldstein, J., and Argov, S. (2004). Possible common biomarkers from FTIR microspectroscopy of cervical cancer and melanoma. Journal of Microscopy, 215(1), 86-41.

Nandiyanto, A. B. D., Fadhlulloh, M. A., Rahman, T., and Mudzakir, A. (2016). Synthesis of carbon nanoparticles from commercially available liquified petroleum gas. IOP Conference Series: Materials Science and Engineering, 128(1), 012042.

Nandiyanto, A. B. D., Putra, Z. A., Andika, R., Bilad, M. R., Kurniawan, T., Zulhijah, R., and Hamidah, I. (2017). Porous activated carbon particles from rice straw waste and their adsorption properties. Journal of Engineering Science and Technology, 12, 1-11.

Nandiyanto, A. B. D., Andika, R., Aziz, M., and Riza, L. S. (2018a). Working Volume and Milling Time on the Product Size/Morphology, Product Yield, and Electricity Consumption in the Ball-Milling Process of Organic Material. Indonesian Journal of Science and Technology, 3(2), 82-94.

Nandiyanto, A. B. D., Oktiani, R., Ragadhita, R., Sukmafitri, A., and Zaen, R. (2018b). Amorphous content on the photocatalytic performance of micrometer-sized tungsten trioxide particles. Arabian Journal of Chemistry, in press.

Paluszkiewicz, C., and Kwiatek, W. M. (2001). Analysis of human cancer prostate tissues using FTIR microspectroscopy and SRIXE techniques. Journal of Molecular Structure, 565, 329-334.

Rigas, B., Morgello, S., Goldman, I. S., and Wong, P. T. (1990). Human colorectal cancers display abnormal Fourier-transform infrared spectra. Proceedings of the National Academy of Sciences, 87(20), 8140-8144.

Rigas, B., and Wong, P. T. (1992). Human colon adenocarcinoma cell lines display infrared spectroscopic features of malignant colon tissues. Cancer Research, 52(1), 84-88.

Schulz, H., and Baranska, M. (2007). Identification and quantification of valuable plant substances by IR and Raman spectroscopy. Vibrational Spectroscopy, 43(1), 13-25.

Shetty, G., Kendall, C., Shepherd, N., Stone, N., and Barr, H. (2006). Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus. British journal of cancer, 94(10), 1460-1464.

Sukuta, S., and Bruch, R. (1999). Factor analysis of cancer fourier transform infrared evanescent wave fiberoptical (FTIR-FEW) spectra. Lasers in Surgery and Medicine, 24(5), 382-388.

Wang, H., Wang, H. C., and Huang, Y. J. (1997). Microscopic FTIR studies of lung cancer cells in pleural fluid. Science of the Total Environment, 204(3), 283-287.

Wong, P. T., Goldstein, S. M., Grekin, R. C., Godwin, T. A., Pivik, C., and Rigas, B. (1993). Distinct infrared spectroscopic patterns of human basal cell carcinoma of the skin. Cancer Research, 53(4), 762-765.

Wong, P. T. T., Papavassiliou, E. D., and Rigas, B. (1991). Phosphodiester stretching bands in the infrared spectra of human tissues and cultured cells. Applied spectroscopy, 45(9), 1563-1567.

Wood, B. R., Quinn, M. A., Burden, F. R., and McNaughton, D. (1996). An investigation into FTIR spectroscopy as a biodiagnostic tool for cervical cancer. Biospectroscopy, 2(3), 143-153.

Wood, B. R., Quinn, M. A., Tait, B., Ashdown, M., Hislop, T., Romeo, M., and McNaughton, D. (1998). FTIR microspectroscopic study of cell types and potential confounding variables in screening for cervical malignancies. Biospectroscopy, 4(2), 75-91.

Wu, J. G., Xu, Y. Z., Sun, C. W., Soloway, R. D., Xu, D. F., Wu, Q. G., and Xu, G. X. (2001). Distinguishing malignant from normal oral tissues using FTIR fiber-optic techniques. Biopolymers: Original Research on Biomolecules, 62(4), 185-192.

Yang, Y., Sulé-Suso, J., Sockalingum, G. D., Kegelaer, G., Manfait, M., and El Haj, A. J. (2005). Study of tumor cell invasion by Fourier transform infrared microspectroscopy. Biopolymers: Original Research on Biomolecules, 78(6), 311-317.

Yoshida, S., Miyazaki, M., Sakai, K., Takeshita, M., Yuasa, S., Sato, A., Kobayashi, T., Watanabe, S., and Okuyama, H. (1997). Fourier transform infrared spectroscopic analysis of rat brain microsomal membranes modified by dietary fatty acids: possible correlation with altered learning behavior. Biospectroscopy, 3(4), 281-290.



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