Fermented Grain Beverage Supplementation Following Exercise Promotes Glycogen Supercompensation in Rodent Skeletal Muscle and Liver

Tsubasa Shibaguchi, Rie Ishizawa, Atsushi Tsuji, Yuya Yamazaki, Keizo Matsui, Kazumi Masuda


The purpose of this study was to investigate the effects of post-endurance exercise fermented grain beverage (FGB) supplementation on glycogen reaccumulation in rat skeletal muscle and liver. Twelve-hour fasted male Wistar rats, 10-week-old, performed five 30-min bouts of swimming separated by 5-min rest periods in order to deplete tissue glycogen storage. The rats were orally administrated either water, glucose, or FGB in 30, 60, 90, and 120 min after the exercise. Immediately and/or 240 min after the exercise, soleus and gastrocnemius muscles and liver were removed and analyzed. A large glycogen reaccumulation was observed in skeletal muscles and liver at 240 min after the exercise when either glucose or FGB were ingested. This response tended to be greater in FGB-treated than in glucose-treated animals, particularly in liver and gastrocnemius muscle. These results suggest that post-endurance exercise FGB supplementation enhances glycogen restoration in both skeletal muscle and liver.


Fermented Grain Beverage; Rodent Skeletal Muscle; Glycogen Supercompensation; Liver

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Bergström, J. & Hultman, E. (1966). Muscle glycogen synthesis after exercise: an enhancing factor localized to the muscle cells in man. Nature, 210(5033), 309-310.

Bergström, J., Hermansen, L., Hultman, E., & Saltin, B. (1967). Diet, muscle glycogen and physical performance. Acta physiologica scandinavica, 71(2), 140-150.

Doi, M., Yamaoka, I., Fukunaga, T., & Nakayama, M. (2003). Isoleucine, a potent plasma glucose-lowering amino acid, stimulates glucose uptake in C2C12 myotubes. Biochemical and biophysical research communications, 312(4), 1111-1117.

Fuchs, C. J., Gonzalez, J. T., Beelen, M., Cermak, N. M., Smith, F. E., Thelwall, P. E., Taylor, R., Trenell, M. I., Stevenson, E. J., & van Loon, L. J. (2016). Sucrose ingestion after exhaustive exercise accelerates liver, but not muscle glycogen repletion compared with glucose ingestion in trained athletes. Journal of applied physiology, 120(11), 1328-1334.

Kanda, Y. (2013). Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone marrow transplantation, 48(3), 452-458.

McGuinness, O. P. & Cherrington, A. D. (2003). Effects of fructose on hepatic glucose metabolism. Current opinion in clinical nutrition and metabolic care, 6(4), 441-448.

Morifuji, M., Kanda, A., Koga, J., Kawanaka, K., & Higuchi, M. (2010). Post-exercise carbohydrate plus whey protein hydrolysates supplementation increases skeletal muscle glycogen level in rats. Amino acids, 38(4), 1109-1115.

Nilsson, L. H. & Hultman, E. (1973). Liver glycogen in man – The effect of total starvation or a carbohydrate-poor diet followed by carbohydrate refeeding. Scandinavian journal of clinical and laboratory investigation, 32(4), 325-330.

Nishitani, S., Matsumura, T., Fujitani, S., Sonaka, I., Miura, Y., & Yagasaki, K. (2002). Leucine promotes glucose uptake in skeletal muscles of rats. Biochemical and biophysical research communications, 299(5), 693-696.

Rose, A. J. & Richter, E. A. (2005). Skeletal muscle glucose uptake during exercise: how is it regulated?. Physiology, 20(4), 260-270.

Saitoh, S., Yoshitake, Y., & Suzuki M. (1983). Enhanced glycogen repletion in liver and skeletal muscle with citrate orally fed after exhaustive treadmill running and swimming. Journal of nutritional science and vitaminology, 29(1), 45-52.

Sakamoto, K. & Holman, G. D. (2008). Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic. American journal of physiology-endocrinology and metabolism, 295(1), E29-E37.

DOI: https://doi.org/10.17509/ijost.v2i1.5985


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