The Effect of Drying Methods on the Quality of Snake Grass (Clinacanthus Nutans) and Its Potential as Chocolate Filling

Mansoor Abdul Hamid, Lim Kai Yen, Ai Nurhayati, Oslida Martony, Maryana Mohamad Nor, Wahyu Nurkholis Hadi Syahputra

Abstract


This study investigated the effect of cabinet and vacuum drying methods on the antioxidant activity of snake grass (Clinacanthus nutans) and evaluated its potential as a functional chocolate filling. Vacuum drying at 40°C significantly preserved higher total phenolic content (TPC) and DPPH radical-scavenging activity (p<0.05) than cabinet drying. Four chocolate filling formulations incorporating 0–150 g of snake grass powder were developed and assessed for sensory quality, proximate composition, and storage stability over 8 weeks. Formulation F2 (100 g snake grass powder) was identified as optimal, yielding above-average sensory scores, the highest acceptable antioxidant capacity, and an improved nutritional profile (7.25% moisture, 30.61% fat, 5.41% protein, 52.61% carbohydrates) with superior storage stability compared to the control. These findings demonstrate that vacuum drying is the preferred processing method for preserving the bioactive compounds of snake grass, and that its incorporation into chocolate filling at 100 g per formulation produces a functional product with both acceptable quality and enhanced nutritional value.

Keywords


Antioxidant, Clinacanthus nutans, Functional chocolate, Storage stability, Vacuum drying

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Akther, S., Jothi, J. S., Badsha, M. R., Rahman, M. M., Das, G. B., & Alim, M. A. (2023). Drying methods effect on bioactive compounds, phenolic profile, and antioxidant capacity of mango powder. Journal of King Saud University-Science, 35(1), 102370. https://doi.org/10.1016/j.jksus.2022.102370

Ban, W. K., Fong, I. L., Khong, H. Y., & Phung, J. H. Y. (2022). Wound healing, antimicrobial and antioxidant properties of Clinacanthus nutans (Burm. f.) Lindau and Strobilanthes crispus (L.) Blume extracts. Molecules, 27(5), 1722. https://doi.org/10.3390/molecules27051722

Barek, M. L., Hasmadi, M., Zaleha, A. Z., & Fadzelly, A. M. (2015). Effect of different drying methods on phytochemicals and antioxidant properties of unfermented and fermented teas from Sabah Snake Grass (Clinacanthus nutans Lind.) leaves. International Food Research Journal, 22(2), 661–670.

Bazyar, Y. Z., Rabbani, M., & Azizi, M. H. (2025). Effect of Ganoderma lucidum to Produce Functional Chocolate: Physicochemical, Textural and Sensory Properties. Food Science & Nutrition, 13(9), e70676. https://doi.org/10.1002/fsn3.70676

Calín-Sánchez, Á., Lipan, L., Cano-Lamadrid, M., Kharaghani, A., Masztalerz, K., Carbonell-Barrachina, Á. A., & Figiel, A. (2020). Comparison of traditional and novel drying techniques and its effect on quality of fruits, vegetables and aromatic herbs. Foods, 9(9), 1261. https://doi.org/10.3390/foods9091261

Chen, C. S., Loke, C. F., Poh, T. V., & Tan, S. P. (2024). Phytochemical screening, antioxidant properties, and photocytotoxicity of Clinacanthus nutans leaf extracts. Vegetos, 37(4), 1652-1661. https://doi.org/10.1007/s42535-023-00731-0

Das, A., Parashar, D. P., Raychaiudhuri, U., & Chakraborty, R. (2022). Studying the effect of different drying methods on phenolic content, antioxidant activity, color and antimicrobial activity in Assam tea (Camellia assamica). Journal of Plant Biochemistry and Biotechnology, 31(3), 615-624. https://doi.org/10.1007/s13562-021-00753-2

Faccinetto-Beltrán, P., Gómez-Fernández, A. R., Santacruz, A., & Jacobo-Velázquez, D. A. (2021). Chocolate as carrier to deliver bioactive ingredients: Current advances and future perspectives. Foods, 10(9), 2065. https://doi.org/10.3390/foods10092065

Horwitz, W., & Latimer, G. W. (Eds.). (2005). Official methods of analysis of AOAC International (18th ed.). AOAC International.

Hřivna, L., Machálková, L., Burešová, I., Nedomová, Š., & Gregor, T. (2021). Texture, color, and sensory changes occurring in chocolate bars with filling during storage. Food science & nutrition, 9(9), 4863-4873. https://doi.org/10.1002/fsn3.2434

Hu, D., Liu, X., Qin, Y., Yan, J., Li, R., & Yang, Q. (2023). The impact of different drying methods on the physical properties, bioactive components, antioxidant capacity, volatile components and industrial application of coffee peel. Food Chemistry: X, 19, 100807. https://doi.org/10.1016/j.fochx.2023.100807

International Organization for Standardization. (2005). Sensory analysis — Methodology — Paired comparison test (ISO Standard No. 5495). https://www.iso.org/standard/31621.html

Jusoh, H. M., & Haron, N. (2019). A total phenolic content and their antioxidant activity of clinacanthus nutans extract. International Journal of Allied Health Sciences, 3(4), 904-913.

Mella, C., Vega-Gálvez, A., Uribe, E., Pasten, A., Mejias, N., & Quispe-Fuentes, I. (2022). Impact of vacuum drying on drying characteristics and functional properties of beetroot (Beta vulgaris). Applied Food Research, 2(1), 100120. https://doi.org/10.1016/j.afres.2022.100120

Mouhoubi, K., Boulekbache-Makhlouf, L., Madani, K., Palatzidi, A., Perez-Jimenez, J., Mateos-Aparicio, I., & Garcia-Alonso, A. (2022). Phenolic compounds and antioxidant activity are differentially affected by drying processes in celery, coriander and parsley leaves. International Journal of Food Science and Technology, 57(6), 3467-3476. https://doi.org/10.1111/ijfs.15670

Mustika, A., Fatimah, N., Safitri, I., Susanti, N., & Noor, N. S. (2023). Clinacanthus nutans L extracts reduce the serum tumor necrosis factor-α, malondialdehyde, and interleukin-6 levels and improve the langerhans islet area in diabetic rat models. Clinical Medicine Insights: Endocrinology and Diabetes, 16, 11795514231196462. https://doi.org/10.1177/11795514231196462

Ng, K. S., Tan, S. A., Bok, C. Y., Loh, K. E., Ismail, I. S., Yue, C. S., & Loke, C. F. (2022). Metabolomic approach for rapid identification of antioxidants in Clinacanthus nutans leaves with liver protective potential. Molecules, 27(12), 3650. https://doi.org/10.3390/molecules27123650

Pothitirat, W., Chomnawang, M. T., Supabphol, R., & Gritsanapan, W. (2009). Comparison of bioactive compounds content, free radical scavenging and anti-acne inducing bacteria activities of extracts from the mangosteen fruit rind at two stages of maturity. Fitoterapia, 80(7), 442-447.

Samanta, S., Sarkar, T., Chakraborty, R., Rebezov, M., Shariati, M. A., Thiruvengadam, M., & Rengasamy, K. R. (2022). Dark chocolate: An overview of its biological activity, processing, and fortification approaches. Current Research in Food Science, 5, 1916-1943. https://doi.org/10.1016/j.crfs.2022.10.017

Sarıtaş, S., Duman, H., Pekdemir, B., Rocha, J. M., Oz, F., & Karav, S. (2024). Functional chocolate: Exploring advances in production and health benefits. International Journal of Food Science and Technology, 59(8), 5303-5325. https://doi.org/10.1111/ijfs.17312

Żbik, K., Górska-Horczyczak, E., Onopiuk, A., Kurek, M., & Zalewska, M. (2023). Vacuum and convection drying effects on volatile compounds profile and physicochemical properties of selected herbs from Lamiaceae family. European Food Research and Technology, 249(10), 2569-2581. https://doi.org/10.1007/s00217-023-04309-7




DOI: https://doi.org/10.17509/mpgk.v18i1.99646

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