A novel In-situ Enzymatic Cleaning Method for Reducing Membrane Fouling in Membrane Bioreactors (MBRs)

M. R. Bilad, M. Baten, A. Pollet, C. Courtin, J. Wouters, T. Verbiest, Ivo F J. Vankelecom


A novel in-situ enzymatic cleaning method was developed for fouling control in membrane bioreactors (MBRs). It is achieved by bringing the required enzymes near the membrane surface by pulling the enzymes to a magnetic membrane (MM) surface by means of magnetic forces, exactly where the cleaning is required. To achieve this, the enzyme was coupled to a magnetic nanoparticle (MNP) and the membrane it self was loaded with MNP. The magnetic activity was turned by means of an external permanent magnet. The effectiveness of concept was tested in a submerged membrane filtration using the model enzyme-substrate of Bacillus subitilis xylanase-arabinoxylan. The MM had almost similar properties compared to the unloaded ones, except for its well distributed MNPs. The enzyme was stable during coupling conditions and the presence of coupling could be detected using a high-performance anion-exchange chromatography (HPAEC) analysis and Fourier transform infrared spectroscopy (FTIR). The system facilitated an in-situ enzymatic cleaning and could be effectively applied for control fouling in membrane bioreactors (MBRs).


Membrane bioreactor (MBR); Fouling; Magnetic membrane; Magnetic nanoparticle; In-situ membrane cleaning; Enzyme immobilisation

Full Text:



Badday, A.S., Abdullah, A.Z., and Lee, K. (2014) Artificial neural network approach for modeling of ultrasound-assisted transesterification process of crude Jatropha oil catalyzed by heteropolyacid based catalyst. Chemical engineering, and processing: process intensification, 75, 31–37.

Farobie, O., and Matsumura, Y. (2015a) A comparative study of biodiesel production using methanol, ethanol, and tert-butyl methyl ether (MTBE) under supercritical conditions. Bioresource technology, 191, 306–311.

Farobie, O., and Matsumura, Y. (2015b) Biodiesel production in supercritical methanol using a novel spiral reactor. Procedia environmental sciences, 28(SustaiN 2014), 204–213.

Farobie, O., and Matsumura, Y. (2015c) Effect of pressure on biodiesel production in supercritical tert butyl methyl ether (MTBE). Journal of the japan institute of energy, 94, 755–762.

Farobie, O., and Matsumura, Y. (2015d) Effectiveness of spiral reactor for biodiesel production using supercritical t-butyl methyl ether (MTBE). Journal of the japan petroleum institute, 58(2), 110–117.

Farobie, O., Sasanami, K., and Matsumura, Y., (2015e) A novel spiral reactor for biodiesel production in supercritical ethanol. Applied Energy, 147, 20–29.

Farobie, O., Hasanah, N., and Matsumura, Y. (2015f) Artificial neural network modeling to predict biodiesel production in supercritical methanol and ethanol using spiral reactor. Procedia environmental sciences, 28(SustaiN 2014), 214–223.

Farobie, O., Yanagida, T., and Matsumura, Y. (2014) New approach of catalyst-free biodiesel production from canola oil in supercritical tert-butyl methyl ether (MTBE). Fuel, 135, 172–181.

Frusteri, F., Arena, F., Bonura, G., Cannilla, C., Spadaro, L., and Di Blasi, O. (2009) Catalytic etherification of glycerol by tert-butyl alcohol to produce oxygenated additives for diesel fuel. Applied catalysis A: General, 367(1), 77-83.

Moradi, G. R., Dehghani, S., Khosravian, F., and Arjmandzadeh, A. (2013) The optimized operational conditions for biodiesel production from soybean oil and application of artificial neural networks for estimation of the biodiesel yield. Renewable energy, 50, 915-920.

Pasqualino, J.C., Montane, D., and Salvado, J. (2006) Synergic effects of biodiesel in the biodegradability of fossil-derived fuels. Biomass and bioenergy, 30, 874–879.

Saka, S., and Kusdiana, D. (2001) Biodiesel fuel from rapeseed oil as prepared in supercritical methanol. Fuel, 80, 225–231.

DOI: https://doi.org/10.17509/ijost.v1i1.2211


  • There are currently no refbacks.

Copyright (c) 2016 Indonesian Journal of Science and Technology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Journal of Science and Technology is published by UPI.
StatCounter - Free Web Tracker and Counter
View My Stats