Phylogenetic Analysis of Bengkulu Citrus Based on DNA Sequencing Enhanced Chemistry Students' System Thinking Skills: Literature Review with Bibliometrics and Experiments

Nadia Amida, N. Nahadi, Florentina Maria Titin Supriyanti, L. Liliasari, Devri Maulana, Rendi Zulni Ekaputri, Indri Sari Utami

Abstract


The study aims to enhance students' system thinking skills through phylogenetic analysis based on DNA sequencing results. The study is carried out through three steps: (i) pretest: evaluate students' knowledge, (ii) treatment: analysis of affinity relationships through phylogenetics using DNA sequence data, and (iii) postest: measurement of improvement in systems thinking skills after implementation. Evaluations are conducted using written tests as well as worksheets and supported by surveys, questionnaires, and interviews for qualitative analysis. The experiment began with the isolation of citrus DNA, determination of concentration and purity, amplification, reading of sequencing results, and phylogenetics. The results of the teaching analysis show that there is a significant improvement in students' mastery of conceptual and system thinking skills, and there is a high correlation between the pretest and posttest. The problem analysis of interviews and questionnaires is also done to see the enthusiasm for activities as well as the effectiveness of the worksheets used. The study comes with new information on determining the relationship between local citrus in Bengkulu and improving the students' system thinking skills.

Keywords


Citrus; DNA isolation; Phylogenetic analysis; Sequence DNA; System thinking skills

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References


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DOI: https://doi.org/10.17509/ijost.v9i2.67813

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